ABSTRACT
One of the most vital considerations in choosing probiotic bacteria is their propensity to adhere to biotic and abiotic membranes. The majority of the microorganisms with probiotic characteristics come from lactic acid bacteria. In both humans and animals, Lactobacillus species make up a sizeable portion of the microbiota in many bodily regions, including the female reproductive system and the digestive system. Solubilizing agents are utilized to promote a drug's solubility when it is weakly soluble. The purpose of the study is to investigate the concentration of solubilizing agents that will optimally desorb lactobacillus reuteri, lactobacillus gasseri, and lactobacillus rhamnosus from the adsorbent. Tween 80 was prepared in varying concentrations (100%, 75%, 50%, 25, 12.5%) to investigate its ability in the removal/desorption of already adhered lactobacillus species from cotton wool, cellulose, charcoal, kaolin, zeolite after incubation at 370 c for 48hours in MRS medium. The study showed that a 75% concentration of tween 80 had the best desorption yield of lactobacillus reuteri, lactobacillus gasseri, lactobacillus rhamnosus the adsorbents used. Keywords: desorption, tween 80, solubilizing agent, adsorbents, lactobacillus reuteri, lactobacillus gasseri, and lactobacillus rhamnosus.
