ABSTRACT
Background: Probiotic bacteria, such as Lactobacillus species, exhibit adhesion to various surfaces. Tween 20, a solubilizing agent, is recognized for its potential in dislodging these adhered bacteria. Objective: This study seeks to assess the efficacy of Tween 20 in detaching adhered Lactobacillus reuteri, Lactobacillus gasseri, and Lactobacillus rhamnosus from solidified egg albumen and egg membranes. Methods: Various concentrations of Tween 20 were applied to solidified egg albumen and egg membranes previously incubated with Lactobacillus reuteri, Lactobacillus gasseri, and Lactobacillus rhamnosus. Results: The investigation revealed that a 50% concentration of Tween 20 yielded the highest desorption of Lactobacillus gasseri and Lactobacillus rhamnosus from the respective adsorbents, while Lactobacillus reuteri required higher concentrations of 75% and 100% for maximum desorption. This concentration proved optimal for the desorption and quantification of the target bacteria from solidified egg albumen and egg membranes. Conclusion: Tween 20 effectively desorbed Lactobacillus species (gasseri, rhamnosus, reuteri) from both solidified egg albumen and egg membranes, enabling accurate quantification. Notably, Lactobacillus reuteri exhibited a higher optimal desorption concentration (75%-100%) compared to L. gasseri and L. rhamnosus (50%), suggesting potential differences in surface properties or interactions with the adsorbents. This research provides valuable insights for optimizing desorption methods in studies of Lactobacillus adhesion. Keywords: Desorption, Tween 20, Quantification, Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus rhamnosus, Egg Albumen, Egg Membranes
