ABSTRACT
Probiotics are defined as living organism and when administered in adequate amount improve the health. Adhesion is an important mechanism of action of probiotics. The term Adhesion refers to the ability of an organism, such as a probiotic, to attach to a surface, such as the lining of the gut. It also increases the opportunity to interact with the host resulting, In a temporary colonization increasing their transit time in the gut to exert their intended beneficial effect. This research seeks to understand the effect of different concentration on Lactobacillus reuteri, Lactobacillus gasseri and Lactobacillus rhamosus using different adsorbent. The growth inhibitory effect of Tween 20 was studied. It was observed that Tween 20 had no inhibitory effect on the growth of Lactobacillus reuteri, Lactobacillus gasseri and Lactobacillus rhamosus. Quantitative experiment was also carried out to determine the deadhesion effect of Tween 20. It was observed that de- adhesion yield of tween 20 from Lactobacillus reuteri ranged from (8.4 x 105 to 1.3 x109 cfu/ml), (5.0x 105 to 2.8 x 108 cfu/ml), 91.4x106 to1.7 x 109 cfu/ml), (1.1 x106 t0 1.5 x109 cfu/ml), (1.6 x106 to 1.1 x 109 cfu/ml), from cellulose, charcoal, cotton wool, kaolin and zeolite respectively as adsorbent. The highest yield was of 1.7 x 109 cfu/ml was observed at a concentration of 25% of the adsorbent cotton wool. For Lactobacillus gasseri it ranged from (4.0x102 to 3.3x106 cfu/ml), (1.4x105 to 4.1x107 cfu/ml), (4.6x103 to 4.5x107 cfu/ml), (6.8x105 to 5.3x107 cfu/ml), (1.24x106 to 1.3x108 cfu/ml), from cellulose, charcoal, cotton wool, kaolin and zeolite respectively as adsorbent. The highest yield was of 4.5 x 108 cfu/ml was observed at a concentration of 0% of the adsorbent cotton wool. For Lactobacillus rhamosus, it ranged from (1.3x106 to 1.5x105 cfu/ml), 8.4x104 to 1.6x109 cfu/ml), (1.4x105 to 1.33x1010cfu/ml), 4.2x105 to 1.14x109 cfu/ml), (5.6x106 to 1.7x109 cfu/ml) from cellulose, charcoal, cotton wool, kaolin and zeolite respectively as adsorbent. The highest yield was of 1.33x 1010 cfu/ml was observed at a concentration of 25% of the adsorbent cotton wool
