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ABSTRACT
The nutritional and financial advantages of palm oil, had make its uses grown, driving up its price and encouraging adulteration under the guise of maintaining quality. Azo dyes are widely used in a wide range of economic sectors, including the food, textile, and cosmetics industries. Due to potential harm to health, Sudan III and Sudan IV are especially concerning. This study aims to evaluate the toxicological effects of Sudan III and Sudan IV azo dyes on palm oil, paying special attention to alterations in body weight, liver function, and the liver tissues' Catalase and Superoxide Dismutase enzymatic activity. Male rats were orally administered Sudan III and Sudan IV azo dyes in various amounts in palm oil, for a predetermined amount of time. Liver function tests, body weight measurements, and enzymatic assays were used to assess the impact of the azo dyes. In comparison to groups that were not exposed to the azo dyes Sudan III and Sudan IV, the results demonstrated significant changes in liver health indices, body weight, and enzyme activity in rats exposed to these dyes. These findings highlight the potential toxicological risks associated with ingesting palm oil contaminated with Sudan III and Sudan IV azo dyes, understanding the necessity of regulating and supervising the areas of food safety and public health.