THE STUDY OF THE DESORPTION EFFECT OF SOLUBULIZING AGENTS ON ADHERED LACTOBACILLUS REUTERI

ABSTRACT

There are different methods used in experimentally assessing microbial adhesion. They include the direct method and indirect  methods. The direct methods are laborious and the bacteria have to remain culture able. while the indirect method are less have low through put ,less accurate, less sensitive.In this experiment, the desorption effect of solubilizing agents(propylene glycol and tween 80) on adhered Lactobacillus reuteri was studied. .My aim was to observe the effect of contact time and different concentrations (100%, 75%,50%,25%,12.5%) of solubilizing agents (Propylene glycol and Tween 80) on the growth of Lactobacillus reuteri. The growth inhibitory effect different concentrations (100%, 75%,50%,25%,12.5%) of solubilizing agents (Propylene glycol and Tween 80) on the growth of Lactobacillus reuteri was determined at different contact time.A growth inhibitory test and then we went further to determine the determine the desorption effect of the solubilizing agents by testing the effect of different contact time (2 ½ minutes to 30 minutes) and different concentrations of the solubilizing agents on Lactobacillus.

 The result of this experiment showed that the solubilizing agents used had no growth inhibitory effect on the adhered lactobacillus reuteri and so would not affect the validity of the experiment. The desorption effect Tween 80 and Propylene glycol were not concentration dependent but can be said to be time dependent at an optimum desorption concentration( which was 25% in this experiment).Tween 80 had the highest desorption effect with a yield of at a contact time of 15 minutes at 25% concentration.