ABSTRACT
The investigation into the induction of metabolic syndrome by a high fructose diet is a critical focus in health research. The rising occurrence of metabolic disorders has prompted exploration into potential interventions. This study delves into the effectiveness of cellgevity and specific polyherbal supplements (paxherbal bitters and yoyo cleanser bitters) in mitigating the impacts of a high fructose diet on metabolic parameters in rats. The experiment spanned 28 days and involved Thirty (30) male Wistar rats, each weighing an average of 200 g, divided into six groups of five rats each. The control group received a standard diet and water. Group two to six were subjected to a high fructose diet along with 10% fructose water. Additionally, specific interventions were administered to these groups: Atorvastatin (0.57 mg/kg-bw) to group three, Paxherbal bitters (600 mg/kg-bw) to group four, Yoyo cleanser bitters (600 mg/kg-bw) to group five, and Cellgevity (26.15 mg/kg-bw) to group six. After the experimental period, the rats were sacrificed, and blood samples were collected for analyzing metabolic parameters. Histopathological examinations of liver, heart, and kidney tissues were conducted to evaluate structural changes and potential protective effects of the experimental supplements. The resulting data from biochemical analysis underwent statistical analysis using one-way ANOVA (Analysis of Variance) and the multiple comparison Tukey’s test for significance. The high fructose diet with 10% fructose water caused significant (P < 0.05) alterations in metabolic markers and histopathological changes indicative of vascular, cardiac, hepatic, and renal damage. Atorvastatin, Paxherbal bitters, Yoyo cleanser bitters, and Cellgevity displayed noteworthy efficacy in mitigating these adverse effects, showcasing varying degrees of protective potential across different parameters. Paxherbal bitters, Yoyo cleanser bitters, and Cellgevity significantly (p < 0.05) prevented the elevation in Fasting Blood Glucose, Insulin, Homeostatic Model Assessment of Insulin Resistance index, total cholesterol, triglycerides, Low Density Lipoprotein – cholesterol (LDL-Cholesterol), Very Low Density Lipoprotein – cholesterol (VLDL-Cholesterol), and atherogenic index of plasma levels which were elevated in negative control group. They also significantly (p < 0.05) prevented High sensitive C - Reactive protein elevation and maintained total antioxidant capacity, with the bitters and Cellgevity significantly reducing Malondialdehyde levels compared to the negative control group. Moreover, they significantly (p < 0.05) reduced the elevated sodium and uric acid levels and prevented increases in Alanine aminotransferase, Aspartate aminotransferase, Total bilirubin, and unconjugated bilirubin levels, with Yoyo cleanser bitters even lowering Alanine aminotransferase levels below the normal control. Only Cellgevity significantly increased conjugated bilirubin levels. Histopathological examination revealed severe cardiovascular, hepatic, and renal tissue damage in rats on a high fructose diet, which was prevented from happening in varying degrees by Paxherbal bitters, Yoyo cleanser bitters, and Cellgevity. This research underscores the potential of herbal bitters and Cellgevity in mitigating the harmful effects of metabolic syndrome inducing high fructose diet, indicating a promising avenue for therapeutic interventions against high fructose-induced metabolic syndrome and organ damage.
