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ABSTRACT
The study evaluated the effect of aqueous: Ethyl Acetate fraction of Detarium microcarpum stem bark extract on sickle cell. Aqueous: Ethyl Acetate fraction (1:4;v/v) of Detarium microcarpum stem bark was obtained using the standard protocols of a step wise fractionation that employs the use of a separating funnel. The activities of aqueous: Ethyl Acetate fraction of D. microcarpum stem bark was assessed in vitro on blood samples obtained from sickle cell patients. Ethical clerarance and informed consent form was obtained prior to collection of the blood samples. Blood samples were obtained from these patients by venipuncture and collected into EDTA tubes. The samples were subjected to paper chromatography analysis to confirm the HbSS status of the patients. The samples were prewashed by centrifugation at 1000rpm for 10min with phosphate buffered saline until a clear transparent top layer was obtained. The packed red blood cells were treated with sodium metabisulphite to induce sickling in vitro.
