ABSTRACT

This study was divided into three phases the acclimatization phase, the induction phase and the treatment phase. The acclimation phase was done to settle the rats into the environment and each group given a diet of 100g normal feed, this phase lasted 14 days. The induction phase was done to induce anemia on the rats through a low protein diet (LPD). A total of 25 rats were used and was divided into 5 groups of 5 rats each. Group 1 was taken as normal control all through the course of study and Group 2 was taken as the PEM control, a diet plan of 100g normal feed was given to Group 1 and 50g LPD given to the other four groups, this feeding plan continued for six weeks, this was done to induce anaemia and oxidative stress. After six weeks of induction, the treatment phase started and MIM and leaf extract were administered to the rats. MIM was administered to Group 3, MCAP 100 mg/kg bw was administered to Group 4, while MCAP 250 mg/kg bw  was administered to Group 5. At this stage, all the rats were fed normal diet except the untreated anemic rats (PEM control). After four weeks of treatment, the rats were sacrificed and their liver, kidney and spleen were excised, blotted, weighed and homogenized in ice-cold normal saline. Blood was also taken in EDTA containers for haematological assessment. The results upon treatment reveals an increase in SOD in the kidney and spleen and a reduction in GSH and MDA in the liver and spleen when compared to the negative control (group 2).