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ABSTRACT
Exposure to radiation (Artificial and Natural) has been on the increase with the advent of new technology and modern society. Artificial ionizing radiation is known for its importance in several areas of life (for e.g. X-ray in medicine and industries), although they are important, they are also known for their deleterious effect in the body tissues leading to associated health degradation. The purpose of this study is to investigate the effect of x-rays on the concentration of calcium and magnesium ions in the aqueous humour of bovine, caprine and porcine eyes. The reason for this choice of animals was due to their similarity with human anatomy/physiology and their distinct rearing environments. In this study, carefully enucleated bovine eyes were obtained from certified cows freshly slaughtered in government abattoir at Ikpoba Slope, Benin City, Edo state. Carefully enucleated caprine eyes were obtained from certified goats freshly slaughtered at Animal Market along Siluko road, Benin City, Edo state. Carefully enucleated porcine eyes were obtained from certified pigs freshly slaughtered in Animal Market along Siluko road, Benin City, Edo state. The eyes were transferred to the Radiology department, Health center in the University of Benin, Benin-City, Edo state, where they were exposed to X-rays with an x-ray machine. After irradiation, the aqueous humour samples were collected. The aqueous humour samples were then transferred to Plant Biology and Biotechnology department in the University of Benin, where they were analyzed for Calcium (Ca2+) and Magnesium (Mg2+) concentration using an atomic absorption spectrometer. Statistical analysis showed no significant effect of different doses of x-ray on the ionic concentration of Calcium and Magnesium in the aqueous humour of bovine, caprine and porcine eyes. The was also no significant difference on the effect of different dosesof x-ray across the three groups of specimens.
