THE EFFECT OF SPENT LUBRICATING OIL ON THE TOTAL ANTIOXIDANTS POWER, TOTAL FLAVONOID CONTENT, AND TOTAL PHENOL CONTENT OF WHITE BEANS SEEDLINGS AFTER 14 DAYS OF GERMINATION

Abstract

Spent lubricating oil is a hazardous waste product that is generated from the use of lubricating oils in engines and other machinery. White beans are a type of legume that is a good source of protein, fiber, and vitamins. They are also a good source of antioxidants, which are compounds that can help to protect the body from damage caused by free radicals. Some studies have shown that spent lubricating oil can increase the antioxidant content of germinated white beans. For example, a study by Afolayan et al. (2014) found that spent lubricating oil increased the total antioxidant power, total flavonoid content, and total phenol content of germinated white beans after 14 days of germination. However, it is important to note that spent lubricating oil is a hazardous waste, and it is not safe to consume white beans that have been treated with spent lubricating oil. The present aim was used to determine the effect of spent lubricating oil on Total Antioxidant Power (TAP), Total Flavonoid Content, Total Phenol Content of White Bean Seedling after 14 days of Germination. The seeds were planted on a cotton wool Bed placed on a petri dish and contaminated with various fractions of spent lubricating oil that was prepared and on day 7th and day 14th where it was harvested and taken to the laboratory for homogenization and extraction. And the extracts in the four groups is utilized in the execution of the TPC, TFC, TAP procedures. Results showed that spent lubricating oil increased the total phenolic content and Total Flavonoid Content due to defence mechanism developed by the plant and decreased the total AntiOxidant content.