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ABSTRACT
Jatropha tanjorensishas garnered attention for its potential bioactive compounds with diverse pharmacological properties.Understanding the molecular mechanisms by which Jatropha tanjorensis or its bioactive compounds modulate KLF-1 and GATA-1 factors can provide valuable insights into its potential therapeutic applications. The objective of this study was to determine the effects of Jatropha tanjorensis on KLF-1 and GATA-1 genes in mediating erythropoiesis in male albino Wistar rats. A total of thirty (30) male adult albino Wistar rats were selected into five (5) groups. The groups were control group, phenyl hydrazine group, 500mg/Kg of Jatropha tanjorensisgroup,1000mg/Kg of Jatropha tanjorensisgroup and 2000mg/Kg of Jatropha tanjorensisgroup. RNA extraction And semi-quantitative polymerasechainreaction (PCR)wasused to isolate RNA from rat bonemarrow, Complimentary DNA wassynthesized and wassubsequentlyamplifiedusingpolymerasechainreaction. Gel electrophoresiswasused to determine the bands of the genes. The findings revealed thatthe mRNA expression mRNA expression of KLF-1 was significantly higher in the phenylhydrazine treated group when compared to control (p<0.05). Groups 3 showed significantly lower expression of KLF-1 when compared to control group and untreated group (p<0.05). Groups 4 showed significantly lower expression of KLF-1 when compared to the untreated group (p<0.05). Groups 5 showed significantly higher expression of KLF-1 when compared to the control group (p<0.05). Group 3, 4 and 5 showed significant difference from each other. The mRNA expression of GATA-1 was significantly higher in the phenylhydrazine treated group when compared to control (p<0.05). Groups 3 and 5 showed significantly higher expression of GATA when compared to control group and untreated group (p<0.05). Groups 4 and 5 showed significant difference from each other. In conclusion, Jatropha tanjorensisexerted a concentration-based increase and decrease in xii KLF-1 and GATA-1 expressions. These results suggest that Jatropha tanjorensis could influence erythropoiesis by modulating the expression of KLF-1 and GATA-1.
