ABSTRACT
This study investigated the effect of aqueous-methanol pulp extract of Azanza garckeana on the liver function of Wistar albino rats and the histopathological assessment of the liver. Azanza garckeana is a plant commonly found in Africa, it’s consumed locally and reportedly used for diverse medicinal purposes. A total of twenty four (24) rats were used for this study. The rats were acclimatized for fourteen (14) days and divided into four groups withfive(5) rats for each group. Group 1 served as the control group and group 2 to 4 were the experimental test groups administered doses of 50mg/kg, 300 mg/kg, and 2000mg/kg of Aqueous-methanol pulp extract daily for fourteen days respectively. At the end of the experimental period, the rat were anaethesized with chloroform, and blood sample was collected from the heart and the abdominal aorta, the rats were then sacrificed. Liver function analysis were then carried out using standard procedures and methods . The results of the 50mg/kgB.wt, 300mg/kgB.wt, and 2000mg/kgB.wt experimental group animals relative to the control group revealed a non-significant increase (P>0.05) in alanine transaminases, total bilirubin, conjugate bilirubin, total protein, albumin and globulin. This result also revealed a significant decrease (P<0.05) in the aspartate transaminase level, with a significant change (P<0.05) in the 50mg/kgB.wt and 300mg/kgB.wt in the alkaline phosphatase level, while the results of the 50mg/kgB.wt, 300mg/kgB.wt, and 2000mg/kgB.wt experimental group animals of the female Wistar albino rats relative to the control group revealed a non-significant increase (P>0.05) in alanine transaminases, total bilirubin, conjugate bilirubin, total protein, albumin and globulin. This result also revealed a significant decrease (P<0.05) in the aspartate transaminase level, with a significant change (P<0.05) in the 50mg/kgB.wt and 300mg/kgB.wt in the alkaline phosphatase level as well. Conclusively, aqueous-methanol pulp extract of Azanza garckeana fruits has no negative impact on the liver.
