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ABSTRACT
This project was designed to study the impact of aqueous extract of Fragaria ananassa on sperm analysis parameters in adult male wistar rats, aiming to discern the potential effects of Fragaria ananassa bioactive compounds on sperm count, motility and morphology. A total of twenty (20) adult male wistar rats were used for this study. The rats were divided into four (4) experimental groups (A-D) with five (n=5) rats in each group. The rats were acclimatized for two weeks before commencement of administration. Group A was the control group. Group B, C and D were administered 50mg/kg, 100mg/kg and 200mg/kg body weight of Fragaria ananassa extract orally via gavage. The rats were anesthetized using chloroform vapor and semen was collected by ligation of two ends of the vas deferens for sperm analysis. The result was statistically analyzed using Graph-pad prism version 9.5. Comparison between groups were done using one-way ANOVA. The results were presented as mean ±SEM and p-value less than 0.05 (p<0.05) was considered statistically significant. The result from the study revealed significant increase in sperm count in group C and D when compared to the control group (group A) and insignificant changes in sperm motility and morphology in group B, C and D when compared to the control group (group A). In conclusion aqueous extract of Fragaria ananassa demonstrated a significant positive effect on sperm count in adult male wistar rat and does not have significant effect on sperm motility and morphology of adult male wistar rat. While this finding hold promise for individuals with low sperm count, it also highlights the complexity of male fertility, further research is necessary to elucidate the underlying mechanism responsible for increase in sperm count and to determine if this effect translates to enhanced fertility.
