ABSTRACT

Datura stramonium, a plant in the Solanaceae family, contains tropane alkaloids that are known for their pharmacological and toxic effects. The aim of this study was to determine how varying concentrations of the plant extract influence liver protein synthesis and overall liver function, given the liver's role in detoxification and metabolism.A total of 12 samples and 480 fruit flies were divided into 4 groups: A, B, C and D representing control, 2000 mg/kg, 4000 mg/kg, and 8000 mg/kg concentrations respectively. Survival analysis revealed a significant decrease in survival rates as the extract concentration increased. The control group maintained a survival rate of 94.74% throughout the study, while the 8000 mg/kg group experienced complete mortality by day 21. Th data generated ware analyzed and compared between the various groups and the control using an appropriate statistical tool (SPSS version 27) employing a one-way ANOVA. The result showed no significant change in total protein levels (p=0.752), but notable, concentration-dependent changes were observed in albumin and globulin levels. Albumin levels were highest at 2000 mg/kg (0.80 ± 0.10) and declined with higher concentrations (p=0.009), while globulin levels decreased at 2000 mg/kg (0.43 ± 0.15) and increased at higher doses (p=0.002). A strong negative correlation between albumin and globulin (R = -0.93, p = 0.000) suggests a compensatory relationship between these proteins. These results demonstrate the selective modulation of protein synthesis by D. stramonium leaf extract, providing important insights into its broader physiological effects. The observed dose-dependent patterns indicate that lower concentrations may promote protein synthesis, while higher concentrations may inhibit certain protein fractions. In conclusion, this study offers valuable information about the biochemical interactions of D. stramonium and its potential applications in protein metabolism and immune regulation. Future studies should focus on identifying the specific bioactive compounds within D. stramonium leaf extract responsible for the observed dose-dependent modulation of albumin and globulin levels.