SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES BY CELLFREE ENZYMES OF Ochrobactrum intermedium

ABSTRACT

Nanoparticles are environment-friendly due to their reduced level of toxicity, higher biodegradability, selectivity and improved efficacy in their performance even at extreme conditions of pH, temperature and salinity, Metal nanoparticles (Nps) are the subject of much research interest in recent decades due to their unique optical properties. This study is aimed at the characterization and synthesizing of metallic nanoparticles by the cell-free enzymes of Ochrobactrum intermedium. The methods used in the synthesis and characterization of nanoparticles are UV-VIS spectroscopy, FTIR(Fourier Transform Infrared Spectroscopy), XRD(X-ray Diffraction), SEM and EDX(Scanning Electron Microscope and Energy Dispersive X-ray Spectroscopy), UV-VIS spectra showed the absorption peak formed at 400nm.FTIR showed an increase in transmittance at 413cm1 and 416cm-1 . The XRD spectrum exhibited peaks at 19.000 (210),21.920 (122),23.000 (111),25.240 (200),29.950 (231),34.000 (142),47.980 (241),56.000 (22 0),59.970 (311) and 63.980 (222). The EDX spectrum showed that synthesized NPs had a particle size of 28.52nm and consist of high amounts of Ag (67.65%), carbon(16.65%) and oxygen(9.84%). Synthesized NPs applied as an antimicrobial agent against clinical isolates showed the highest inhibition against Escherichia coli and Staphylococcus aureus. In conclusion, the antimicrobial properties of silver nanoparticles were found to be effective against some bacteria.