SUSCEPTIBILITY PROFILE OF ANAEROBIC BACTERIAL OBTAINED FROM PLAQUE FROM DENTAL PATIENT IN THE DENTAL CLINIC

ABSTRACT

Background: Dental plaque is a complex biofilm harboring a diverse microbial community, including anaerobic organisms, which play a pivotal role in oral dental and systemic xiii infection. This study analyzes the anti-microbial susceptibility profile of all anaerobic isolates obtained from study participants to antimicrobial agents frequently used in the study centre. Methodology: The study evaluated a minimum of 105 specimens obtained from plaque deposits in the mouths of enrolled study participants who presented for scaling and polishing at the study center. The susceptibility test was carried out using multi-antibiotics susceptibility disc containing the following classes of antibiotics such as fluroquinolones, macrolides, aminoglycosides, cephalosporins, antifolates and penicillin’s. Multi antibiotics containing disc were placed onto the culture media and incubated at 37 °C for 24 hours. The positive control Petri dishes did not have antibiotics incorporated into the Mueller-Hinton agar plate. All plates were incubated at 37 °C for 24 hours in duplicate. Inhibition zone diameters were obtained and appropriately recorded. These were interpreted as susceptible or resistant by comparison with published guidelines for antimicrobial susceptibility testing for commonly occurring pathogens, as obtained from clinical isolates (EUCAST, 2015). Results: The bacterial isolates obtained showed good susceptibility to the fluroquinolones, macrolides, aminoglycosides and cephalosporins antibiotics while having low susceptibility towards penicillin’s and antifolate drugs. Conclusion: Monitoring the susceptibility profiles of anaerobic bacteria from dental plaque is crucial in identifying emerging resistance patterns. This data can guide healthcare providers in adapting treatment strategies and developing new therapeutic approaches.