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On the International Union for Conservation of Nature (IUCN) list, Desplatsia dewevrei serves as one of the endangered plant species. Micropropagation is also known as in vitro multiplication, also referred to as tissue culture even though, there’s a slight difference which shall be discussed later. They all connotes the same meaning which is the rapid multiplication of plant for various purposes so, micropropagation, in vitro multiplication and tissue culture were used interchangeably in this report. The aim of this study was to develop a protocol for the micropropagation of Desplatsia dewevrei, which is an endangered medicinal plant. The explants used were leaves, stalks and seed. The leaves and stalk were collected from fresh plantlets while the seed was gotten from the dried fruit of the plant. They were thoroughly subjected to different sterilization procedures to prevent or reduce contamination. Murashige and Skroog media was used supplement with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) and with different hormonal concentrations to determine the most efficient hormonal concentration requirement. The concentrations of NAA used were 0.1, 0.2, 0.5 and 1.0 ppm, each in combination with 0.25 ppm of BAP. Some of the culture got contaminated which were discarded however, we were able to establish a protocol with the non-contaminated cultures. NAA concentrations of 0.2, 0.5 and 1.0 ppm each in combination with BAP concentration of 0.25 ppm each performs better. Replicate C - (0.5 ppm NAA + 0.25 ppm BAP) produced the best callus while replicate D - (1.0 ppm NAA + 0.25 ppm BAP) has more effect on the elongation of stalk. During the four weeks of taking data, seed have not started giving any sign of development which may be due to the fact that seeds takes longer period to produce callus. Apart from the hormonal concentration, from the results obtained from this study, it is also established that leaves, stalk and seeds may require different concentrations of or duration of exposure to sterilizing reagents.
