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ABSTRACT
The depletion of fossil fuel reserves and the environmental concerns associated with their use have driven the search for sustainable biofuels. This study explores the potential of cassava bagasse as a viable lignocellulosic feedstock for biobutanol production via Simultaneous Saccharification and Fermentation (SSF). Acid pretreatment was employed to enhance enzymatic hydrolysis by reducing lignin content and increasing cellulose accessibility, as confirmed through XRD, FTIR, and SEM analyses. The hydrolysis process was optimized using a synergistic enzyme cocktail comprising cellulase, betaglucosidase, and pectinase, with Response Surface Methodology (RSM) determining the optimal enzyme concentrations for maximum sugar release. The optimized SSF conditions, including pH 5.5, an inoculum size of 20% (v/v), and a temperature of 30°C, resulted in a significant improvement in biobutanol yield, demonstrating the efficiency of process integration. Enzyme synergy analysis revealed that beta-glucosidase and pectinase exhibited the highest degree of interaction, enhancing hydrolysis efficiency. Despite these advancements, challenges such as enzyme cost and process scalability remain critical barriers to industrial implementation. Future research should focus on cost-effective enzyme production, in situ product recovery techniques, large-scale process validation, and microbial strain optimization to improve butanol tolerance and productivity. A comprehensive techno-economic analysis is also necessary to assess commercial viability. This study establishes a strong foundation for the advancement of biobutanol production from corn cob, contributing to the development of sustainable biofuels.
