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ABSTRACT
The production of amylase, a vital enzyme used in numerous sectors, has historically involved a number of steps. The aim of this research was to investigate the production of amylase by Aspergillus niger using corn cobs as a substrate. The fungus was isolated from a rotting corn cob and used to inoculate the medium. The corn cob was processed and subjected to heat and acid pretreatment. Control samples had no treatments applied. Fermentation was carried out using the various pretreated corn cob for 12 days and samples analyzed at time intervals were changes in pH, reducing sugar concentrations, cell biomass and amylase. The results revealed that the control medium had the highest dry fungal biomass (g/L) and the heat-pretreated corn cob had the highest amylase yield (µg/mL), with values of 13.85 and 115.73, respectively. After 12 days of fermentation, the amylase yield for the control medium and heat-pretreated corn cob was lower, at 65.23µg/mL and 66.42µg/mL respectively. The corncob that had been acid-pretreated had the least amount of dry fungal biomass. This study offers insights into the production of amylase, the effects of the various fermentation parameters, and the efficiency of the substrate used.
