ABSTRACT

Mobile phones, door handles and pens are inanimate objects while hands are animate which act as vehicles in the transmission of pathogens to humans. With the growing regards over their role as potential origin of pathogens reservoir, hence the need for this study to determine the bacterial contamination of different samples.  Seventy (70) samples which include fifteen (15) from hands, fifteen (15) from mobile phones, twenty five (25) from door handles, fifteen (15) from pen, all selected within Wellspring University were swabbed using sterile swab stick and there after analyzed using standard microbiological methods. In the procedure, the cotton swab stick was immersed in sterilized normal saline water and was pour into different sterilized test tube. All isolates were characterized and identified based on their cultural morphological and biochemical characteristics. Six (6) bacterial isolates were identified from the samples: Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus auerus, Bacillus subtilis, Escherichia coli, and Streptococcous pyrogenes.  Escherichia coli had the highest percentage prevalence of 30.0%. Streptococcous pyrogenes had the least percentage prevalence of 6.7%. Some isolates such as Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis were resistant to cefexime and cefuroxime. However, majority of the isolates were significantly susceptible to ofloxacin, ciprofloxacin and erythromycin. It is therefore obvious that, the high level of microbial contaminants of these animate and in-animate object causes great danger to the public health. Effort should be made to regularly disinfect surfaces.