ABSTRACT
Most people across the world utilize medicinal plants to cure a variety of ailments. Kigelia africana (Bignoniaceae), often known as the African sausage tree, is a medicinal plant that has traditionally been used to treat a variety of ailments. The plant is regarded as having a wide range of therapeutic properties, including antidiabetic, anticancer, antibacterial, analgesic and anti-inflammatory, antioxidant, and so on. This study describes Kigelia africana's phytochemical, pharmacological, and membrane stabilizing properties. This experiment is designed to assess the anti-inflammatory properties of Kigelia africana fruit. The in-vitro anti-inflammatory activity of Kigelia africana (Lam.) Benth. extract and its fractions was investigated using stabilization of human red blood cell membrane lysing and inhibition of hemolysis technique, which includes heat-induced and hypotonicity-induced hemolysis. Phytochemically, methanol plant extract (MPE) and its fraction (EF) showed positive results for the presence of many phytochemical components such as saponins, tannins, flavonoids, cardiac glycosides, etc. The ethyl acetate (EAF) and n-hexane fractions produced reactions indicating the presence of flavonoids saponins, terpenoids, cardiac glycosides, and phlobatannins. The extract and various fractions demonstrated concentrationdependent membrane stability that was comparable to that of the standards (Diclofenac sodium and Aspirin). The methanol extract, ethylacetate and DCM fraction of Kigelia Africana fruit, at concentrations of 500μg/ml considerably prevented erythrocyte membrane lysis induced by hypotonic solution with percentage mean inhibition of 31.232%, 23.810%, and 41.422% respectively. The methanol extract, DCM and n-hexane fraction showed positive result for stabilization of HRBC membrane by heat-induced hemolysis with highest percentage mean inhibition of 27.275%, 36.217% and 23.552% at 1000μg/ml concentration (Table 3.3), compared to Aspirin with a beneficial impact for with highest percentage mean inhibition of 27.068% at the xi same concentration. The ethylacetate fraction showed highest percentage mean inhibition of 26.168% at 100μg/ml (Table 3.3).Methanol extract of Kigelia africana showed promising antiinflammatory action in-vitro in a concentration-dependent manner. The fruit plant Kigelia africana has promising anti-inflammatory properties.
