You have no items in your shopping cart.
ABSTRACT
Food poisoning is caused by food that looks, smells, and tastes normal. Because the customer is uninformed that there is a potential problem with the food, they consume a large amount of it and become ill. The aim of this study was to determine the phenotypic and molecular detection and multidrug resistant genes present in Thaumatococcus daniellii Leaf-wrapped ready to eat rice in Benin metropolis. A total of nine samples were purchased from three markets. Each sample was tested for the presence of microorganisms using Pour plate method on appropriate selective media. Analysis obtained showed the Total Heterotrophic count ranged from 13.8 to 18.0×102 cfu/g, Total Escherichia coli count ranged from 2.55 to 14.5 ×101 cfu/g and Total Staphylococcal count from 4.0 to 6.0×102cfu/g. Four isolates were obtained; Staphylococcus aureus, Bacillus cereus, Escherichia coli and Klebsiella spp. Antibiotic susceptibility test was determined using disk diffusion method. The antibiotic susceptibility profile of the isolates showed they were 100% resistance to Ceftazidime, Cefuroxime, Cefixime, Augmentin, 96.5% resistance to Nitrofurantoin, Erythromycin, Cloxacillin, Ceftriaxone and 65.5% resistance to Gentamicin. Aminoglycoside resistance genes; aac (6) gene and ant (4) gene were detected using polymerase chain reaction and the biofilm forming activity for the isolates was 34.4%. The occurrence of these bacteria isolate constitutes public health risk on consumers. Therefore, personal hygiene should be implemented, washing of the leaves with clean water before serving the food and regular inspection by food sanitary authorities.
