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ABSTRACTThis study was designed to examine the relationship between Organizational Culture and the performance of Business Organizations (Banks) in Benin City, Edo State, Nigeria. In examining the relationship, Organization’s Culture component such as sense of mission in the organization, consistency of organization culture, organizational adaptability and employee’s involvement were correlated with organization’s performance component such as organization’s growth, employee’s commitment, goal achievement and customer’s satisfaction. The study used data collected from research questionnaires and the data were analyzed using percentage and Pearson’s product moment correlation analysis. A total of two hundred (200) questionnaires were distributed two hundred respondents in banks in Benin City, Edo State. One hundred and eighty seven (187) copies of the questionnaires were duly filled and returned. Results of hypothesis test with correlation coefficient values (r) of 0.50 and above were accepted and indicated positive and significant relationship. The findings of the study revealed that sense of mission in the organization, consistency of organization culture and organizational adaptability were found to have positive and significant relationship with organization’s growth, but with exception to customer’s satisfaction. In view of the findings of the study, it is recommended that there should be training programmes for managers and employees of UBA Bank, Eco-Bank, Guaranty Trust Bank and Zenith Bank on how to improve organization’s product / service delivery to boost customer’s satisfaction. Also, management of the Organizations should adopt effective communication system across all level of the organization especially when communicating messages on employee’s performance expectation and organization’s goals and objectives.