ABSTRACT
Background: Probiotics, beneficial live organisms, exert positive effects on host health when administered appropriately. Adherence of probiotic bacteria to various surfaces is crucial for their efficacy, with lactic acid bacteria being prominent in probiotic formulations. Lactobacillus species, abundant in human and animal microbiota, colonize diverse body sites, notably the digestive and female reproductive systems. Objective: This study aimed to determine the optimal concentration of Propylene Glycol for desorbing Lactobacillus reuteri, Lactobacillus gasseri, and Lactobacillus rhamnosus from adsorbents. Methods: Propylene Glycol solutions were prepared at varying concentrations (100%, 75%, 50%, 25%, 12.5%) to assess their efficacy in desorbing Lactobacillus species from solidified egg albumen and egg membrane. Incubation at 37°C for 48 hours in MRS medium facilitated bacterial adherence. Results: Analysis revealed that Propylene Glycol concentrations of 25%, 12.5%and 25% exhibited the highest desorption yield of Lactobacillus reuteri, Lactobacillus gasseri, and Lactobacillus rhamnosus from the tested adsorbents. Conclusion: This study underscores the potential of Propylene Glycol, particularly at 25% and 12.5% concentrations, as an effective desorbing agent for adhered Lactobacillus species. Such findings contribute to enhancing probiotic applications, particularly in optimizing bacterial adhesion and release processes. Keywords: desorption, propylene glycol, solubilizing agent, adsorbents, Lactobacillus reuteri, Lactobacillus gasseri, and Lactobacillus rhamnosus.
