Abstract
Background : Bisphenol A (BPA) is an ubiquitous chemical contaminant that has recently been associated with adverse effects on human health. BPA exposure impairs synaptic transmission and contributes to BPA-induced cognitive impairment in the brain; BPA tremendously elevates oxidative stress and Alpha Beta in the cortex and hippocampus of the Alzheimer's diseased brain. This study was designed to investigate the neuro-toxic effects of bisphenol-A and the possible interventional role of rutin using drosophila melanogaster. Methods: 540 flies were randomized into 6 groups of 30 flies each and were treated as follows for 15 days: Control ( on 9.8g of basal diet), Bisphenol-A (BPA, 1mm), Rutin (2.5mg), Rutin (5mg), BPA + Rutin ( 1mm BPA, 2.5mg Rutin), BPA + Rutin ( 1mm BPA, 5mg Rutin). Survival research was performed to determine the best Rutin dosage to use for this investigation and the effect of BPA toxicity on the survival of flies. A second group experiment waa carried out for the determination of negative geotaxis, open field and biochemical assays. 480 flies were randomized into 4 groups of 40 flies each and were treated as follows for 6 days: control ( on 9.8g of basal diet), Bisphenol-A (BPA, 1mm), Rutin (2.5mg), BPA + Rutin ( 1mm BPA, 2.5mg Rutin). At the end of the experimental period the files were homogenized and the supernatant used for biochemical assays- SOD,GST,MDA,CAT and AChE. Result: BPA significantly reduced the survival rate, groups treated with BPA+RUTIN showed increased climbing activity of flies, elevated MDA concentration and AChE activities of flies. SOD, CAT and GST activities were also significantly reduced (p<0.05) in BPA treated flies. In the co treatment protocol, SOD and the climbing activity of the flies were improved.
Conclusion this research shows that RUTIN possesses antioxidant and anticholinesterase properties and could be of therapeutic benefits against BPA induced toxicity.
