MOLECULAR IDENTIFIFCATION OF MICROORGANISMS ISOLATED FROM POULTRIES IN BENIN CITY

ABSTRACT

This study was carried out to evaluate the antibiotic susceptibility pattern of shigella species isolated from feed, water and droppings sampled from poultries within Benin City, Edo State. The samples were collected from four (4) poultries (Ugborroad axis, Sapele road axis, Ekenwan road axis (trade fare) and Evboriaria road axis) between August and October, 2020. The results showed that for poultry feeds sampled, mean aerobic count for feeds ranged from17.95±3.04 (poultry C) to28.2±1.55 (poultry B) while Shigella-Salmonella count ranged from 2.32 ± 0.84(Poultry C) to 8.30±1.27 (poultry A).For Poultry water, mean total heterotrophic count ranged from 0.85±0.49 for poultry A to 1.85±0.35 for poultry B. There was noSalmonella- Shigella counts obtained in any poultry water except poultry B which had values ranging from 0.25 ± 0.07 to0.30±0.22.For Poultry dung, mean total aerobic count ranged from 11.90 ± 2.96 for poultry C to 36.35±4.17 for poultry B. while mean Salmonella-Shigella count ranged from6.05±1.06 for poultry C to 14.75±6.29 for poultry B. Twenty-seven (27) bacteria organisms were isolated and characterized biochemically for similarity to Shigella species from all samples collected. Antibiotic susceptibility screening revealed that all isolates were resistant to Ceftazidime (CAX), Cefixime (CXM) and Cefuroxime (CRM),partially resistant toGentamicin (GEN), Augumentin (AUG) Ciprofloxacin (CPR) and Ofloxacin (OFL). The isolates were however 100% susceptible to Nitrofurantoin. Sequencing of 16s RNA of three (3) isolates identified the organisms as Providencia rettegeri (2) and Klebsiella pneumoniae (1). This study showed the public health importance of isolates from poultries as multidrug resistant organisms. There should therefore be strict regulation of the use of antibiotics in animals to minimize the emergence of resistant bacteria in animals which may further aggravate the public health problem associated with dissemination of antibiotic resistant bacteria into the environment.