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ABSTRACT
A crude oil contaminated soil remediation was investigated using cow dung manure which is an organic waste from animal droppings for stimulating indigenous microbial activities with the view of optimizing their efficiency using RSM. The crude oil was weathered by exposure to the atmospheric condition for two weeks with occasional stirring after which it was stored for further. The initial concentration of the crude oil was determined using the UV/VIS spectrophotometer which will be used for sample analysis for the determination of THC. The soil samples were air-dried, homogenized, sieved through a pore size of 2mm to remove dirt and suspended particles and stored at room temperature in a polyethylene bag. Standard procedures were used to characterize soil samples for physicochemical and microbiological characteristics. The pH was obtained by dissolving the soil sample in distilled water and reading off the value with the pH meter. From the the numerical optimization carried out, to maximize THC removal from the soil. The blend of biochar and cow dung gave an optimum condition of 147.784ml of crude oil, 20.007g of biostimulant and 17.408 remediating days which gives THC removal of 95.982% with a desirabilty of 1.000. Biochar only gave an optimum condition of 130.449ml of crude oil, 42.663g of biostimulant and 20.019 remediating days which gives THC removal of 85.901% with a desirabilty of 1.000 while poultry dropping gave an optimum condition of 146.583ml of crude oil, 45.082g of biostimulant and 18.601 remediating days which gives a THC of 93.095% with a desirabilty of 1.000.
