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ABSTRACT
The study was carried out to investigate the microbiological evaluation of hand rails from the University of Benin City, Edo State, Nigeria. The samples were aseptically collected using sterile saline water and sterile swab sticks at designated places in the school. Sampling point at the school served as Faculty of education, Faculty of medicine and Faculty of Engineering. Swab samples were collected from these points and taken to the laboratory for microbiological quality assessment. The microbiological assessment exposed the presence of Escherichia coli, Bacillus cereus, Proteus mirabillis, Citrobacter sp, Staphylococcus aureus, Staphylococcus epidermidis. The colony count of total bacteria in swab samples inoculated on nutrient agar, mannitol salt agar and potato dextrose agar were 0.5-53×102 cfu/g, 0.4-25×102 cfu/g, 0.4-17×102 cfu/g respectively. The most widely distributed bacteria was proteus. The least distributed bacteria was Staphylococcus epidermidis and Staphylococcus aureus. Fungi associated isolated were Fusarium sp and Saccharomyces sp. The fungus of highest occurrence was Saccharomyces sp, while the least distributed was Fusarium sp. The most sensitive antibiotics for Gram positive bacteria was Streptomycin (S) while the least sensitive was Gentamycin (CN). Also for Gram negative bacteria the most sensitive bacteria was Sparifloxacin (SP) while the least was Amoxacillin (AM).This research revealed the prevalence of potential pathogenic bacteria and fungi on hand rails which are capable of causing diseases in the future.
