MICROBIAL ASSESSMENT OF INDOOR AIR OF MICROBIOLOGY LABORATORY IN UNIVERSITY OF BENIN, BENIN CITY

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i h

ABSTRACT

Microbiological quality assessment of indoor air study is one of the most vital investigations to determine the microbial indoor air contaminant. The information on the indoor microbial concentrations of airborne bacteria and fungi is necessary both to estimate the health hazard and to create standards for indoor air quality control. Standard bacteriological methods were used to determine the total bacterial count of the air samples. The bacterial isolates were characterized and identified using morphological and biochemical methods. The percentage distribution and frequency of the isolates were evaluated using statistical method. The results obtained from this study showed that laboratory one had the microbial load of 8.04x104 ± 6.0 Cfu/m3 while laboratory two had the microbial load of 6.85x104± 0.5 Cfu/m3 . Using standard morphological and cultural characterization, the isolates obtained in this study were Staphylococcus aureus, Micrococcus sp, Bacillus subtilis, Streptococcus and Escherichia coli. The Gram positive bacteria were all susceptible to gentamycin and augmentin while the Gram negative showed resistance to these antibiotics. However the isolates were all susceptible to ofloxacin. 

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