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ABSTRACT
Staphylococcus aureus is a Gram-positive, round-shaped bacterium, a member of the Firmicutes, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. The aim of the research was to determine the Staphylococcus aureus isolated from the pigs are methicilin-resistant and to detect the LA-MRSA colonization rate in the pigs. A total number of 12 nasal samples were collected from pigs in Ulemon community and was brought to the laboratory for several laboratory tests with the use of Blood agar and Muller Hinton agar. Enumeration and isolation were done with the use of serial dilution, plating method and streak plating. Observation was made on Characterization and identification of isolated samples and also the cultural and morphological characteristics of the isolates were observed. Several biochemical tests were also carried out on the isolates (such as coagulase, citrate, gram staining, oxidase, indole, catalase, urease, motility antibiotic sensitivity and sugar fermentation test). The bacterial isolates include Staphylococcus aureus, Streptococcus spp, Clostridium difficle, Bacillus subtilis and Acinetobacter spp. The percentage occurrence of S. aureus was 66.7%. Staphylococcus aureus showed resistance to almost all the antibiotics used (cefuroxime, ceftazidime, ceftriaxone, erythromycin, cloxacillin and agumentin) and was highly susceptible to Ofloxacin and Gentamicin. This suggests that Ofloxacin and Gentamicin are antibiotics that are efficient in the treatment of LA-MRSA.
