ISOLATION OF BACTERIA IN MOTHERS GENITALS (NEONATES INFECTION)

ABSTRACT

Escherichia coli and Staphylococcus aureushas been implicated as a common cause of both early and late onset of neonatal infections. The study is aimed at isolating Escherichia coli from neonates and maternal vaginal swab sample from neonates with neonatal sepsis. Samples were collected from neonates with neonatal sepsis using sterile swabs and then transferred immediately to the microbiology laboratory, department of microbiology. University of Benin. Bacterial isolation, enumeration and identification were carried out aseptically using Kirby and Bauer disc diffusion method. A total of 20 isolates were obtained. The isolates were staphylococcus aureus, Escherichia coli, Klebsiellapneumoniae, Lactobacillus,Proteusvulgaris,Pseudomonasaeuriginosahaving the least frequency. The gram positive bacteria had 65% occurrence while the gram negative bacteria had 35% occurrence. 75% of the total bacteria count was implicated in early onset sepsis while 25% was implicated in late onset sepsis and it was discovered that Escherichia coli and staphylococcus aureus had a higher degree of occurrence in early onset sepsis than in late onset sepsis. It was also deduced from this research work that neonatal sepsis occurred more in females than males, the isolated Escherichia coli was discovered to be highly susceptible to Ciprofloxacin, moderately susceptible to Streptomycin and slightly susceptible to Erythromycin but resistant to Chloramphenicol