ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF ENTEROBACTERIACEAE

ABSTRACT

The growing production of poultry as a result of the rising demand for chicken meat has led to a rise in the use of organic wastes (such as chicken manure) as fertilizers. Poultry droppings carries a unique population of pathogenic microorganisms (bacteria, fungi, and viruses) which can cause diseases in poultry, other animals and even humans. This research was carried out to isolate, characterize and identify antibiotic susceptibility pattern of Enterobacteriaceae from poultry droppings in Ovia-Northeast, Benin city, Edo state. Isolation and identification of organisms was done using standard cultural, morphological and biological methods while Extended-spectrum Beta-lactamase (ESBL) production test was done using standard methods. The percentage distribution and frequency of the isolates were evaluated using statistical method. The mean total heterotrophic and coliform bacterial count ranged from 2.95 ± 0.36 x 106 to 5.45 ± 0.42 x 106 cfu/g and 1.09 ± 0.49 x 106 to 3.25 ± 0.25 x 106 cfu/g respectively. The isolates obtained were, Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter freundii, Escherichia coli and Salmonella sp. The antibiotic sensitivity test revealed that most of the isolates found in this study were least susceptible to amoxicillin and were also multidrug resistant strains.