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ABSTRACT
This research delves into the isolation and comprehensive functional group analysis of the butanol fraction obtained from Methanol crude extract of Hibiscus sabdariffa, commonly known as roselle. A portion of the crude methanol extract was screened for phytochemical constituents while the other portion was subjected to vacuum liquid chromatography (VLC) using selected solvent system from 100% hexane, methanol/ acetate (1:1), 100% acetate, 100% methanol and 100% butanol as the eluting solvent. The butanol fraction was dried and characterization was done to ascertain the functional groups by infrared spectroscopy. Alkaloids, saponins and flavonoids were present except phenolics and steroids. The functional groups detected were C-H, Alkyl groups at 1893cm-1 and R-C=O, ester at 1736cm-1. This study suggests that the butanol fraction had phytoconstituents that could be assessed for its medicinal use.
