INVITRO ANTIOXIDANTS AND ANTIDIARRHEAL PROPERTIES OF POLY HERBAL FORMULATED TEA ON MICE

ABSTRACT

A safer and more effective treatment for illnesses has been shown to be polyherbal formulations or therapies. This study aims to explore the antidiarrheal and antioxidant properties of Polyherbal formulated tea (Thespesia garckeana, Curcuma longa, Mucuna pruriens, Musa paradisiaca, and Anthocleista djalonensis). DPPH radical, superoxide radical, hydroxyl radical, and total antioxidant capacity methods were used to study in vitro antioxidant activity. The intestinal transit delay and castor oil-induced diarrhea paradigm in mice was used to evaluate antidiarrhea. The outcome shows that all antioxidants and hydroxyl and superoxide radicals are eliminated by the water-based extract of polyherbal tea. The ascorbic acid-like scavenging action of the Formulated Tea was observed.When compared to the control, the weight of feces was significantly reduced (p<0.05) by the polyherbal-formulated tea (10 and 20 mg/kg). The amount of feces or the time it took for the diarrhea to start were unaffected by the tea. In castor oil-induced diarrhea, loperamide significantly prolonged the time until diarrhea started and reduced the weight and quantity of feces in comparison to the control group (p<0.05). Aqueous extract polyherbal-formulated tea (10mg/kg  and 20 mg/kg) does not affect the percentage of the trip that is made up of charcoal or the distance that charcoal travels in comparison to the control during transit (p > 0.05). Loperamide at 5 mg/kg displays a statistical difference in distance traveled by charcoal and percentage of charcoal travel compared to control (p<0.05). This study revealed that brewing polyherbal (Anthocleista djalonensis, Musa paradisiaca, Mucuna pruriens, Curcuma longa, and Thespesia garckeana) can function as antioxidants. It does not possess antidiarrheal qualities; rather, it may enhance gastrointestinal motility.