ABSTRACT
Oxidative stress plays a pivotal role in the pathogenesis of various diseases, highlighting the need for effective antioxidant interventions. In this study, we investigated the potential of an ethanol extract of Ocimum gratissimum in mitigating cardiac and splenic oxidative stress in rabbits. Our aim was to evaluate the efficacy of the extract in modulating oxidative stress markers and elucidate its underlying mechanisms of action. To achieve this, we conducted a series of experiments involving the extraction and standardization of the Ocimum gratissimum extract, establishment of a reliable oxidative stress model in rabbits, administration of the extract to test groups, and measurement of oxidative stress markers in cardiac and splenic tissues. We assessed biomarkers including catalase, glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), total protein, and nitric oxide. Our results revealed that the Ocimum gratissimum extract demonstrated significant antioxidant properties, as evidenced by its ability to enhance antioxidant enzyme activities and reduce oxidative damage markers in a dose-dependent manner. Specifically, the extract modulated catalase, GPx, SOD, MDA, total protein, and nitric oxide levels in cardiac and splenic tissues, suggesting potential therapeutic benefits in mitigating oxidative stress-induced damage.These findings underscore the therapeutic potential of Ocimum gratissimum extract in combating oxidative stress-related disorders, particularly those affecting cardiac and splenic tissues. Further elucidation of the molecular mechanisms underlying the antioxidant effects of the extract is warranted to optimize its therapeutic applications. Overall, our study contributes valuable insights into the development of novel antioxidant interventions aimed at improving health outcomes and combating oxidative stress-related diseases.
