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Abstract
Annona Muricata L. Popularly known as Soursop is an evergreen plant known for its sweet flavor and medicinal benefits. This study was aimed at investigation of the microbial diversity on trichoderma treated soursop seeds. The specific objectives were to ascertain the percentage infection of microorganisms on the treated seeds, identify and isolate microorganisms associated with the treated seeds and to determine the microbial diversity on treated soursop seeds. The experiment was carried out at the Crop Science Laboratory, Department of Crop science, Faculty of Agriculture and Laboratory, University of Benin, Benin city, Edo state Nigeria. The incubation technique (agar plate method) was employed during the course of the study. Soursop seeds were chopped into tiny bits, surface sterilized and placed on filter paper to absorb moisture. Samples were cultured on Potato Dextrose Agar and Nutrient Agar (PDA/NA). Microbial identifications were done using morphological and biochemical tests for bacteria, while microscopic and cultural characteristics were observed for fungal growth. Three microorganisms were isolated at the conclusion of the investigation, namely Bacillus sp, Staphylococcus sp and Trichoderma. Found as seed samples were treated and taking into account the nature of the microorganisms, it can be deduced that all three microorganisms can be found naturally occurring in the environment. Bacillus subtilis induce systemic resistance and improve seed germination. Staphylococcus sp. is not primarily considered a seed pathogen, however, its presence can have adverse effects on human health when seeds are consumed. Trichoderma reduces the occurrence of plant diseases. Thoroughly washing soursop before consumption is essential to avoid potential health concerns. Further studies should be carried out to understand the benefits of treating soursop seeds/soils with Trichoderma. Additional research should be done to identify adequate preservation techniques.
