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ABSTRACT
Copper (Cu2+) and iron (Fe2+) are essential trace elements (ETEs) for several biological processes such as cell respiration, erythrocyte maturation, and antioxidant defense. However, when dys-homeostasis mechanism of these metals occurs, they are cytotoxic and capable of generating highly damaging free radicals such as hydroxyl radicals via the Fenton or Haber-Weiss reactions, contributing to oxidative stress. The goal of this study was to ascertain if resveratrol may help Drosophila melanogaster oxidative stress caused by iron and copper sulphate. Adult wild type flies were fed Cu2+ and Fe2+ (1mM each) and/or resveratrol (RVS) (30 and 60 mg/kg diet) for 7 days in this investigation. Survival and emergence rate were evaluated by daily recording of flies’ mortality and data were analyzed. Fruit flies wereanaesthesized using CO2 gas, homogenized and centrifuged at 4,000g for 10 minutes at 4 °C. The aliquots of the supernatants were used for the estimation of biochemical markers spectrophotomentric method. The results indicated that flies Co-treated with FeSO4 + CuSO4 (1mM each) + RSV (30 mg/Kg and 60mg/Kg) significantly (p<0.05) ameliorated the elevation of hydrogen peroxide, nitric oxide and lipid peroxidation, acetylchinesterase as well as significant increase (p < 0.05) in GSH, GST, catalase and total thiol when compared with Cu2++ Fe2+ (1mM each) treated group (1.922±0.0587 vs 1.874±0.07901 vs 2.36±0.03315μmol/mg), (6.981±0.4112 vs 5.606±0.7988 vs 7.812±0.3614μmol/mg), (0.1903±0.01555 vs 0.1895±0.02377 vs 0.3148±0.0168nmoles/hr/mg), (0.2538±0.02556 vs 0.2217±0.02009 vs 0.4385±0.02018μmol/min/mg), (5.39±0.4457 vs 5.436±0.5268 vs 4.334±0.3132μmol/mg), (2.008±0.1408 vs 2.195±0.1845 vs 1.393±0.0695μmol/min/mg), (2.903±0.3388 vs 2.795±0.218 vs 1.935±0.164 μmol/min/mg) and (711.1±34.61 vs 745.8±43.14 vs 578.9±9.46μmol/mg) respectively. Flies co-treated with FeSO4 + CuSO4 (1mM each) + RSV (30 mg/Kg and 60mg/Kg) also significantly improved (p < 0.05) eclosion rates and climbing rate when compared with Cu2++ Fe2+ (1mM each) treated group (86.4±13.22 vs 86.4±9.992 vs 33.6±5.543%) and (90.67±3.528 vs 90±1.155 vs 58.67±1.764) respectively. As a result, our data show that resveratrol reduced Cu2+ and Fe2+-induced radical generation in D. melanogaster, and hence could be used in management of disorders involving oxidative stress.
