ABSTRACT
This study aims to investigate the effectiveness of polyherbal mixture of Moringa Oleifera, Syzygium aromaticum, Allium sativum, Vernonia amygdalina, Carica papaya for management of dental pain and inflammation The research was conducted through a series of antimicrobial assays, including the determination of Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentrations (MBCs), alongside measuring zones of inhibition against common oral pathogens such as Staphylococcus aureus, Streptococcus mutans, Lactobacillus acidophilus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The findings revealed that Streptococcus mutans exhibited the lowest MIC at 8mg/mL, indicating a strong antimicrobial effect, while Klebsiella pneumoniae showed the highest MIC at 16mg/mL, suggesting greater resistance to the extracts. The MBC results supported these observations, with Streptococcus mutans also demonstrating the lowest MBC at 18%. Additionally, the study found a concentration-dependent increase in zones of inhibition, highlighting the efficacy of the extracts in inhibiting bacterial growth. Phytochemical analysis indicated the presence of key secondary metabolites, including phenolics, tannins, and flavonoids, with phenolics being the most abundant (295.800 ± 257.550%). Elemental analysis highlighted essential minerals such as calcium (471.831 mg/kg), magnesium, and potassium, which are vital for oral health. Statistical analysis suggested potential synergistic interactions among the extracts, enhancing their overall antimicrobial efficacy. The findings imply that the polyherbal mixture could serve as a natural alternative to synthetic antimicrobial agents in the management of dental pain and inflammation and other oral health conditions, potentially decreasing the risk of malignant transformation associated with chronic inflammation and infection. This research underscores the potential of utilizing natural extracts in oral health management, paving the way for future studies to optimize formulations and explore their clinical applications in treating oral conditions. Future research should focus on clinical trials to validate these findings and explore the long-term safety and effectiveness of the formulation in diverse populations
