In vitro PROPAGATION OF Parkia biglobosa (Jacq) Benth USING NODAL EXPLANTS IN VARYING CONCENTRATIONS OF GROWTH REGULATORS.

ABSTRACT

This study investigated the in vitro propagation of Parkia biglobosa (Jacq) Benth. using nodal explants in Murashige and Skoog medium supplemented with different concentrations of growth regulators. The objective include determination of plant growth regulators that induce shoot proliferation and rapid shoot multiplication from the explants Apical nodes of five weeks old plantlets of Parkia biglobosa were used as explants on Murashige and Skoog medium (MS). The growth regulators applied were combinations of 1-Naphtalene acetic acid (NAA), Kinetin (Kin) and b-benzyl amino purine (BAP) supplemented at 0.25, 0.5, 1.0, 1.5, 2.0 and 2.5 mg/l concentrations for inducing shoot development. Parameters measured include number of shoots, length of shoots, number of roots, length of roots and number of leaves of the cultured explants. Five weeks old nodal part supplemented with 0.5 mg/l KIN and 0.25 mg/l NAA and 1.0 mg/l BAP with 0.25 mg/l NAA gave higher shoot proliferation than other treatments. The control gave least response of shoot development. BAP 1.0 mg/l + NAA 0.25 mg/l with KIN 0.5 mg/l + NAA 0.25 mg/l gave highest shoot length and the lowest according to this research work was the one supplemented with KIN 0.5 mg/l + NAA 0.25 mg/l. BAP 2.0 mg/l + NAA 0.2 mg/l was the combination that generated the highest number of roots while KIN 1.5 mg/l + NAA 0.25 mg/l generated the lowest proliferation rate. BAP 2.0 mg/l + NAA 0.25 mg/l generated the highest root and KIN 1.5 mg/l + NAA 0.25 mg/l generated the shortest root. BAP 1.0 mg/l +NAA 0.25 mg/l generated the highest number of leaves and the control experiment that has no growth regulator generated the lowest number of roots. The result of the study showed that for organogenesis, juvenile nodal cutting serving as explants gave the good response and can be used for rapid propagation of Parkia biglobosa.