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ABSTRACT
Phyllanthus amarus (Schum& Thonn., Euphorbiaceae) is a good antioxidant agent. The purpose of the study was to determine how effective the aforementioned plant is. 32g of the dry leaves were dissolved in 2.5L of ethanol by maceration and an additional 1L with occasional stirring for 4 hours. This planning was done to cover a 72-hour period. After filtering the ethanol extract through a muslin bag, the crude extract was then concentrated in a rotary evaporator until it was dry. The ethanol extract was further assessed for its antioxidant activity by in vitro methods. The in vitro antioxidant activity was evaluated. Some of these assays were done at various concentrations. The result showed that for the FRAP assay, Phyllanthus amarus extract in Chloroform at 200μg/ml (1.04±0.16) had the highest mean transmittance value (p<0.05), and hence has a very effective antioxidant activity, competing favourably with the standard. For determining the Total Antioxidant Capacity, we see that the ethanol, methanol and water extract had a good antioxidant activity, with PE at 200μg/ml better than PW at 800μg/ml and PW at 1000μg/ml when compared to the standard. For the Nitric oxide scavenging Assay, only PM showed a very significant antioxidant activity across all of its varying concentrations (200μg/ml1000μg/ml), with the concentration at 200μg/ml (65.31±0.29) and 1000μg/ml (76.68±0.37) showing an extreme significant antioxidant activity when compared to the standard. The DPPH assay, PM, PW and PE all showed a significant antioxidant activity at varying concentrations, with the concentrations of PM at (200-1000μg/ml) having a better antioxidant activity than the other solvents and standard, which showed a significant antioxidant activity at 1000μg/ml. Reducing Power assay PC at 200μg/ml (0.04±0.00) showed a very good antioxidant activity and competed well with PW at 200μg/ml (0.81±0.08), while PE and Ascorbic acid standard offered no significant antioxidant activity at all, across its varying concentrations.
