ABSTRACT

Bryophyllum pinnatum is a medicinal plant that belongs to the Crassulaceae family. It's traditionally used in Ayurvedic and folk medicine to cure various ailments and its phytochemicals are used in the reduction of metal ions to produce nanoparticles. This study is aimed at the green synthesis of Nickel oxide nanoparticles using Bryophyllum pinnatum as a reducing agent, characterize the synthesized nanoparticles using UV-VIS, SEM-EDX and FTIR analysis and study the microbial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Aspergillus niger, and Candida albicans. The green synthesis of nickel oxide nanoparticles is a safe means of synthesis using Bryophyllum pinnatum leaf extract. This was confirmed by UV-VIS, FT-IR, and SEM-EDX. The UV-VIS showed peaks at 284nm, 244nm, 233nm. This indicated the presence of chromophore. The FTIR result revealed the stretching vibration of the NI-O bonds at 715cm-1. The SEM analysis showed the morphology of the nanoparticles at a magnification of 500X and the EDX revealed that NiO-Nps are composed of oxygen and nickel elements. The antimicrobial analysis carried out against different strains of bacterial and fungi strains, exhibited the tested minimum concentration against all the bacterial strains. The synthesis of NiO NPs is a cost-effective green synthesis route, and it is useful in inhibiting the growth of certain bacteria.