ABSTRACT

Green synthesis methodologies have gained prominence in nanotechnology due to their focus on sustainable and environmentally friendly approaches to nanoparticle production. This study investigates the synthesis of manganese dioxide nanoparticles (MnO₂ NPs) using Moringa oleifera leaf extract and evaluates their antimicrobial properties. The phytochemical screening of Moringa oleifera leaf was carried out using the standard method. This revealed the presence of alkaloids, glycosides, saponins, phenolics, eugenols, and flavonoids in theleaf. The biosynthesised nanoparticle was characterised using UV-VIS, FTIR, SEM-EDX, and XRD. The UV/VIS analysis confirmed the successful formation of MnO₂ NPs, with a characteristic peak observed at approximately 280 nm. FTIR analysis indicated Mn-O interactions on the nanoparticle surface, with no other molecules or functional groups present. SEM-EDX analysis provided insights into the elemental composition of MnO₂ NPs, with manganese being the dominant element. Antimicrobial analysis was observed against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis. There were significant inhibition zones for S. aureus and Candida albicans. Overall, this study highlights the potential of Moringa oleifera-derived materials as effective antimicrobial agents, with implications for biomedical applications and environmental remediation.