ABSTRACT
The expression of Tolllike receptor 4 in rabbits ought to serve as one of the baselines information of TLR4 expression. The gene expression process may be modulated (regulated), including the transcription, RNA splicing, translation, and posttranslational modification of a protein. Regulation of gene expression is the basis for cellular differentiation, development, morphogenesis and the versatility and adaptability of any organism which may therefore serve as a substrate for evolutionary change.
The experiment was carried out to examine the gene expression of tolllike receptor 4 different breeds of rabbits. Forty (40) mixed sex rabbits were used for the experiment, categorized as: New Zealand black (n=10), Hyla white (n=10), Dutch (n=10), and Chinchilla (n=10). About 2ml of blood sample was aseptically collected from the marginal ear vein, into plain vaccutainer tubes containing 3ml of DNA/RNA shield as anticoagulant, using sterile needle of 22 gauge which help to preserve the genetic integrity and expression profiles of the samples. DNA extraction and protocol was carried out using laboratory facilities and equipment at Iqaba biotechnology West Africa Ltd, Ibadan, Oyo State.
Data obtained revealed that that the Actin of sample 4 (Actin NZB) had the highest mean bar level (34.32) as the most expressed monomeric subunit of muscle microfilaments as compared with the mean bar level for other Samples. The TollLike Receptor 4 for NZB breed also had the highest evolutionally divergence innate gene and cellular lipopolysaccharide expression patterns cycle threshold among the four breeds in this study. It was revealed that the Sample 4 (TLR4 NZB) had the most expressed mean bar level rise change due to Sample relative normalized expression compared with other samples. This most evolutionally allelic divergence innate gene and cellular lipopolysaccharide expression patterns cycle threshold of Newzeland Black (NZB) from the other breeds could be ascribed to far phylogenetic tree within the rabbit taxa.
