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ABSTRACTThe increasing dependence on fossil fuels and their adverse environmental impact necessitatesthe development of sustainable alternatives. Biodiesel, a fuel derived from vegetable oils, offersa promising solution. However, using edible oils for biodiesel production raises concerns aboutcompetition with food security. This study explores the viability of a non-edible oil blend (neemand yellow oleander oil) as a feedstock for biodiesel production. To formulate an optimal blendof the two feedstocks for biodiesel production, a D-optimal mixture design was employed on theblends in different ratios. This approach allowed the identification of the blend ratios thatmaximized the oil blend for biodiesel production while improving its properties compared tousing the individual oils. Characterization of both oils individually and as a blend was carried outto determine their properties. The result obtained from the numerical optimizationidentified theoptimal blend of neem seed oil and yellow oleander oil in the proportions 16.15% and 83.85%respectively. The characterization results of the saponification value, acid value, density, andFFA values for the non-edible oils of neem oils were in the range of 350.6 mg KOH/g oil, 29.38– 30.44 mg KOH/g oil, 894 kg/m3, and 14.68% - 15.22% respectively, that of yellow oleanderwere 538.56 mgKOH/g oil, 16.38 – 18.1 mg KOH/g oil, 907 kg/m3, and 8.19% - 9.05%respectively while that of the oil blends were in the range of 322.6 mg KOH/g oil, 18.8 – 29.46mg KOH/g oil, 896 - 905 kg/m3, and 9.40% - 14.73% respectively. These findings demonstratethe potential of utilizing anoptimal blend of neem and yellow oleander oil for sustainablebiodiesel production.