EVALUATION OF THE ROLES OF VITAMIN B COMPLEX AND VITAMIN C ON FEMALE REPRODUCTIVE HORMONES DURING MISALIGNMENT OF NORMAL CIRCADIAN RHYTHM

ABSTRACT

This study evaluates the effects of vitamin B and vitamin C on the hormones of female Sprague-Dawley rats that has undergone altered photoperiodic conditions which includes oestrogen, luteinizing hormone, progesterone and follicle stimulating hormone. These effects were examined with the use of the Enzyme-Linked Immunosorbent Assay (ELISA) technique. The results obtained for vitamin B showed significant changes in the luteinizing and progesterone hormone of the female reproductive system at the dosage of 0.2 mg/kg when compared with the 0.2ml normal saline used for the control (positive and negative). Vitamin C showed significant change in only the luteinizing hormone at the dosage of 3.0 mg/kg in comparison with the 0.2ml normal saline used for the control (positive and negative). The results of this research infers that vitamin B and vitamin C affects the rate of fertility in the female’s reproductive system as a result of their influence on the luteinizing and progesterone hormone, but they do not have significant effect on the oestrogen and follicle stimulating hormone, they therefore don’t have effect on growth of ovarian follicles and regulation of menstrual cycle.