EVALUATION OF THE ATTENUATING EFFECT OF THE COMBINATION OF VITAMIN C, VITAMIN E AND BETA-CAROTENE ON ISONIAZID INDUCED PERIPHERAL NEUROPATHY IN RATS.

Abstract

Isoniazid is commonly utilized in the treatment of tuberculosis according to "direct observed therapy short course" (DOTS), alongside other medications, demonstrating proven effectiveness. Despite its long-standing use of over 70 years, isoniazid has been associated with adverse effects, including the onset of neuropathies. Studies suggest that up to 10% of patients undergoing isoniazid treatment may experience some form of neuropathy. Pyridoxine (Vitamin B6) is the known standard drug used in the treatment of peripheral neuropathy. Vitamin C, Vitamin E and Beta-carotene have shown to have known, little or no effect on peripheral neuropathy. It appears essential to evaluate the potential combined effects of these vitamins to see their level of effectiveness against some symptoms of peripheral neuropathy over Pyridoxine. Established animal models were utilized to examine the impact of the Vitamin combinations on the emergence and advancement of peripheral neuropathic symptoms induced by isoniazid administration. Male Wistar rats were segregated into six groups: De-ionised water, those treated with isoniazid alone, and those receiving combined treatments (isoniazid with Vit C, Vit E and Beta-carotene combination in low, medium, and high daily doses, as well as isoniazid with pyridoxine). Behavioral evaluations, encompassing tests of anxiety, depression, convulsion, sensory and motor function were performed at the conclusion of a 14 days administration. Results indicated that isoniazid administration resulted in a notable decrease in sensory and motor functions, signaling peripheral nerve impairment. The vitamin combinations gave some positive results in some symptoms of peripheral neuropathy. Further studies should be done to ascertain that