EVALUATION OF DNA DAMAGE IN THE AFRICAN CATFISH (Clarias gariepinus) EXPOSED TO A NON-ALCOHOLIC EFFLUENT BY COMET ASSAY TECHNIQUE

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ABSTRACT

This study aimed to examine genotoxicity of a non-alcoholic effluent on the tissues of the African catfish (Clarias gariepinus) using the comet assay (single cell gel electrophoresis) technique. The fish was exposed to different concentrations (0.5, 1.5.3.5 and 5.0%) of the non-alcoholic effluent for 96 hours and a control, and the tissue samples from gills, gonads and liver were analyzed using the comet assay. The results obtained from the comet assay revealed a predominant significant increase in DNA damage (single-strand break) in the liver followed by the gills and gonads which were dose dependent compared to the control. Comet assay proves to be an extremely valuable tool for evaluating genotoxic effects in aquatic organisms, providing perspectives into the potential effects posed by non-alcoholic industrial effluent on the fish tissues.

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