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SUMMARY
This research collates a set of data on pathogen survival, growth, and inactivation during anaerobic digestion (AD) of digestate based on temperature, pH, and lack of oxygen and food waste. However, the amount of the investigated pathogens significantly decreased over the storage time. The ratio of reduction significantly depends on the storage temperatures, pH and also on the Anaerobic digestion composition. While digestion of animal manure is likely to reduce the pathogen burden in digestate for application to land and can reduce environmental contamination inside and outside the household, associated changes in practices may lead to increased exposure to microorganisms derived from dung. To circumvent epidemiological risks, every storage of anaerobic digestion should be considered individually because there are no general biosafety recommendations available that could be applied to all plants and anaerobic digestion. Improved education in hygiene of handling both manures and digestate is needed to maximize the potential benefits of pathogen reduction through anaerobic digestion.