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ABSTRACT
The increasing need for eco-friendly biofuels and biochemicals highlights the importance of converting lignocellulosic biomass, such as corn cobs, into fermentable sugars efficiently. Corn cobs, a plentiful agricultural waste, contain significant amounts of cellulose and hemicellulose, which can be broken down into glucose and other sugars suitable for biofuel production. In order to increase the output of reducing sugars during the hydrolysis of maize cob, this study examines the characteristics of cellulase enzymes. The amount of reducing sugar obtained from the hydrolysis process was measured using the Dinitrosalicylic Acid (DNS) test, and the Bradford Assay Method was used to quantify the amount of enzymes to be employed in protein mass. The results of this study show that, after 6 hours and a percentage hydrolysis of 35.11%, a maximum yield of 2.126g/L of reducing sugar was obtained using 40mg/g of cellulase to hydrolase 5g of corn cob. Similarly, a maximum yield of 4.237g/L of reducing sugar was obtained using 40mg/g of cellulase enzyme cocktail to hydrolse 5g of corn cob after 6 hours and a percentage hydrolysis of 69.96%.
