EFFECTS OF YOYO CLEANSER BITTERS ON GLUCOSE AND INSULIN LEVELS IN MALE WISTAR RATS

ABSTRACT

This study evaluated the effects of Yoyo cleanser bitters on male Wistar rats fed with a high fructose diet. Yoyo Cleanser Bitters, a traditional herbal preparation, is purported to possess potential therapeutic properties for metabolic disorders. Twenty (20) male Wistar rats weighing an average of 200grams each were randomly divided in four groups of five. The rats were fed adlibitum with the feed and clean tap water during the entire course of the experiment (28 days). A basal diet of a standard pelleted grower’s marsh was given to the control group (groupn1), while the treatment diet of 60% high fructose diet along with 10% fructose water was fed to the other three cages. Atorvastatin (0.57mg/kg) was administered daily to group 3 and Yoyo cleanser bitters (600 mg/kg) was administered to the rats in group 4. The treatment were administered using an oro-gastric gavage. Using standard methods, the weekly weight of the rats, their daily food consumption, feed efficiency, and the relative organ weight of the heart, liver and kidney were determined at the end of the 28days study. Comprehensive biochemical assessment of glucose metabolism was performed. The data was analyzed using the one-way analysis of variance (ANOVA), followed by Tukey’s test of significance. A p value of less than 0.05 (p<0.05) was accepted as statistically significant (p<0.05). The results of the study revealed that that Yoyo cleanser bitters significantly reduced (p<0.05) the levels of fasting blood glucose, insulin and HOMA-IR when compared to the group fed a high fructose diet alone. There was no statistically significant difference (p<0.05) between the results of the yoyo cleanser bitters group and the positive control group. The findings from this study indicate that Yoyo cleanser bitters can be said to be have positive therapeutic effects on glucose and insulin levels in metabolic syndrome induced by a high fructose diet.